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mpibr
scic
BrainATUMtome
Commits
99510b87
Commit
99510b87
authored
4 years ago
by
Georgi Tushev
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99510b87
# ATUMtome
UI
User interface to drive
automat
ed tap
e collect
ing ultramicrotome
#
Brain
ATUMtome
control software for an ultramicrotome with an
automat
ic slic
e collect
or
## Tape
[
motor
](
https://www.maxonmotor.de/maxon/view/service_search?query=372161
)
## hardware components
[
driver
](
https://www.maxonmotor.de/maxon/view/product/control/Positionierung/390438
)
### ATUMtome
automated tape collecting
[
ultramicrotome
](
https://www.scienceservices.eu/atumtome-automated-tape-collecting-ultramicrotome.html
)
[
tension motor
](
http://shop.sdp-si.com/catalog/product/?id=A%203Z16-0020C
)
### ultramicrotome
ultramicrotome
[
Leica EM UC7
](
https://www.leica-microsystems.com/products/sample-preparation-for-electron-microscopy/p/leica-em-uc7/
)
RS232
[
interface
](
https://gitlab.mpcdf.mpg.de/mpibr/scic/BrainATUMtome/-/blob/master/config/EMUC7_RS232Interface_OM_EN_09_16.pdf
)
## Parameters
usb camera with
[
OpenCV
](
https://docs.opencv.org/4.5.2/d8/dfe/classcv_1_1VideoCapture.html
)
driver
### Microtome (UC7)
### tape motors
tape motors
[
MAXON 372161
](
https://www.maxongroup.de/maxon/view/service_search?query=372161
)
tension motor
[
A 3Z16-0020C
](
http://shop.sdp-si.com/catalog/product/?id=A%203Z16-0020C
)
microcontroller
[
EPOS2 24/2
](
https://www.maxongroup.de/maxon/view/product/control/Positionierung/390438
)
[
EPOS2
](
https://www.ni.com/de-de/support/downloads/tools-network/download.epos2-positioning-controller-driver.html#374326
)
positioning controller driver
*
maxon motors
*
BOTTOM = USB0
*
TOP = USB1
*
digital outputs
*
GREEN LIGHT
<br>
USB0, 0x0003, DIC_GENERAL_PURPOSE_C, PIN13
*
SOUND
<br>
USB0, 0x0004, DIC_GENERAL_PURPOSE_D, PIN12
*
SUPPORT MOTORS
<br>
USB1, 0x0003, DIC_GENERAL_PURPOSE_C, PIN13
*
RED LIGHT
<br>
USB1, 0x0004, DIC_GENERAL_PURPOSE_D, PIN12
### syringe
## parameters
### microtome (UC7)
*
Cutting speed [mm/s] 0.1 – 2
*
Cutting thickness (feed) [µm] 0.025 – 300
*
Start/Stop cutting Motor off/on
...
...
@@ -22,7 +54,7 @@ User interface to drive automated tape collecting ultramicrotome
*
N/S motor movement [µm] Relative movement (0.1 – 500)
*
E/W motor movement [µm] Relative movement (0.1 – 500)
###
C
amera
###
c
amera
*
Live-image
*
gain
*
exposure time
...
...
@@ -30,52 +62,16 @@ User interface to drive automated tape collecting ultramicrotome
*
Snapshot
*
Video recording
###
W
ater pump
###
w
ater pump
*
1 button to activate pump
*
Dispersed volume/activation: ?? (I will figure it out and send you the values)
###
T
ape mechanism
###
t
ape mechanism
*
Tape speed: 0.1 – 2 mm/s (calibration required!!)
*
Start/stop tape motors
*
Tape tension: support motors on/off
## Implementation
### Classes
*
MainWindow (controller)
*
DriverVideoStream
*
DriverMicrotome
*
DriverWaterPump
*
DriverTapeMechanism
### Virtual class driver
*
DriverAbstract
*
isRunning()
*
Start()
*
Stop()
*
Trigger()
## Drawing a rectangle for ROI
### Classes
*
QGraphicsObject
*
QPen
*
QPainter
*
QGraphicsScene
*
QGraphicsSceneMouseEvent
*
QRectF
## Reference readings
Maxon motor
[
EPOS2
](
http://www.optolab.ti.bfh.ch/wiki/_media/huce:roboticslab:tutorials:maxonmotor:epos_studio_exercises.pdf
)
tutorial
### Maxon Motors
*
BOTTOM = USB0
*
TOP = USB1
### Digital Outputs
*
GREEN LIGHT = USB0, 0x0003, DIC_GENERAL_PURPOSE_C, PIN13
*
SOUND = USB0, 0x0004, DIC_GENERAL_PURPOSE_D, PIN12
*
SUPPORT MOTORS = USB1, 0x0003, DIC_GENERAL_PURPOSE_C, PIN13
*
RED LIGHT = USB1, 0x0004, DIC_GENERAL_PURPOSE_D, PIN12
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