Changed prior initialization

deepof/model_utils.py

@@ -500,62 +500,60 @@ class ClusterOverlap(Layer):
 
         encodings, categorical = inputs[0], inputs[1]
 
-        if tf.test.is_gpu_available():
-
-            hard_groups = tf.math.argmax(categorical, axis=1)
-            max_groups = tf.reduce_max(categorical, axis=1)
-
-            get_local_neighbourhood_entropy = partial(
-                get_neighbourhood_entropy,
-                tensor=encodings,
-                clusters=hard_groups,
-                k=self.k,
-            )
+        hard_groups = tf.math.argmax(categorical, axis=1)
+        max_groups = tf.reduce_max(categorical, axis=1)
+
+        get_local_neighbourhood_entropy = partial(
+            get_neighbourhood_entropy,
+            tensor=encodings,
+            clusters=hard_groups,
+            k=self.k,
+        )
 
-            purity_vector = tf.map_fn(
-                get_local_neighbourhood_entropy,
-                tf.constant(list(range(self.batch_size))),
-                dtype=tf.dtypes.float32,
-            )
+        purity_vector = tf.map_fn(
+            get_local_neighbourhood_entropy,
+            tf.constant(list(range(self.batch_size))),
+            dtype=tf.dtypes.float32,
+        )
 
-            ### CANDIDATE FOR REMOVAL. EXPLORE HOW USEFUL THIS REALLY IS ###
-            neighbourhood_entropy = purity_vector * max_groups
-
-            number_of_clusters = tf.cast(
-                tf.shape(
-                    tf.unique(
-                        tf.reshape(
-                            tf.gather(
-                                tf.cast(hard_groups, tf.dtypes.float32),
-                                tf.where(max_groups >= self.min_confidence),
-                                batch_dims=0,
-                            ),
-                            [-1],
+        ### CANDIDATE FOR REMOVAL. EXPLORE HOW USEFUL THIS REALLY IS ###
+        neighbourhood_entropy = purity_vector * max_groups
+
+        number_of_clusters = tf.cast(
+            tf.shape(
+                tf.unique(
+                    tf.reshape(
+                        tf.gather(
+                            tf.cast(hard_groups, tf.dtypes.float32),
+                            tf.where(max_groups >= self.min_confidence),
+                            batch_dims=0,
                         ),
-                    )[0],
+                        [-1],
+                    ),
                 )[0],
-                tf.dtypes.float32,
-            )
+            )[0],
+            tf.dtypes.float32,
+        )
 
-            self.add_metric(
-                number_of_clusters,
-                name="number_of_populated_clusters",
-            )
+        self.add_metric(
+            number_of_clusters,
+            name="number_of_populated_clusters",
+        )
 
-            self.add_metric(
-                max_groups,
-                aggregation="mean",
-                name="average_confidence_in_selected_cluster",
-            )
+        self.add_metric(
+            max_groups,
+            aggregation="mean",
+            name="average_confidence_in_selected_cluster",
+        )
 
-            self.add_metric(
-                neighbourhood_entropy, aggregation="mean", name="neighbourhood_entropy"
-            )
+        self.add_metric(
+            neighbourhood_entropy, aggregation="mean", name="neighbourhood_entropy"
+        )
 
-            if self.loss_weight:
-                # minimize local entropy
-                self.add_loss(self.loss_weight * tf.reduce_mean(neighbourhood_entropy))
-                # maximize number of clusters
-                # self.add_loss(-self.loss_weight * tf.reduce_mean(number_of_clusters))
+        if self.loss_weight:
+            # minimize local entropy
+            self.add_loss(self.loss_weight * tf.reduce_mean(neighbourhood_entropy))
+            # maximize number of clusters
+            # self.add_loss(-self.loss_weight * tf.reduce_mean(number_of_clusters))
 
         return encodings

deepof/models.py

@@ -473,7 +473,7 @@ class GMVAE:
         # Dummy layer with no parameters, to retrieve the previous tensor
         z = tf.keras.layers.Lambda(lambda t: t, name="latent_distribution")(z)
 
-        if self.number_of_components > 1:
+        if self.number_of_components > 1 and self.overlap_loss:
             z = deepof.model_utils.ClusterOverlap(
                 batch_size=self.batch_size,
                 encoding_dim=self.ENCODING,

supplementary_notebooks/deepof_model_evaluation.ipynb

 %% Cell type:code id: tags:
 ``` python
 %load_ext autoreload
 %autoreload 2
 ```
 %% Cell type:code id: tags:
 ``` python
 import warnings
 warnings.filterwarnings("ignore")
 ```
 %% Cell type:markdown id: tags:
 # deepOF model evaluation
 %% Cell type:markdown id: tags:
 Given a dataset and a trained model, this notebook allows the user to
 * Load and inspect the different models (encoder, decoder, grouper, gmvaep)
 * Visualize reconstruction quality for a given model
 * Visualize a static latent space
 * Visualize trajectories on the latent space for a given video
 * sample from the latent space distributions and generate video clips showcasing generated data
 %% Cell type:code id: tags:
 ``` python
 import os
 os.chdir(os.path.dirname("../"))
 ```
 %% Cell type:code id: tags:
 ``` python
 import deepof.data
 import deepof.utils
 import numpy as np
 import pandas as pd
 import re
 import tensorflow as tf
 from collections import Counter
 from sklearn.preprocessing import StandardScaler
 from sklearn.manifold import TSNE
 from sklearn.decomposition import PCA
 from sklearn.discriminant_analysis import LinearDiscriminantAnalysis
 import umap
 from ipywidgets import interactive, interact, HBox, Layout, VBox
 from IPython import display
 from matplotlib.animation import FuncAnimation
 from mpl_toolkits.mplot3d import Axes3D
 import matplotlib.pyplot as plt
 import seaborn as sns
 import tqdm.notebook as tqdm
 from ipywidgets import interact
 ```
 %% Cell type:markdown id: tags:
 ### 1. Define and run project
 %% Cell type:code id: tags:
 ``` python
 path = os.path.join("..", "..", "Desktop", "deepof-data", "deepof_single_topview")
 trained_network = os.path.join("..", "..", "Desktop", "deepof_trained_weights_280521", "var_annealing")
 exclude_bodyparts = tuple([""])
 window_size = 22
 batch_size = 32
 ```
 %% Cell type:code id: tags:
 ``` python
 %%time
 proj = deepof.data.project(
     path=path, smooth_alpha=0.999, exclude_bodyparts=exclude_bodyparts, arena_dims=[380],
 )
 ```
 %%%% Output: stream
-    CPU times: user 41.9 s, sys: 3.25 s, total: 45.2 s
-    Wall time: 37.5 s
+    CPU times: user 43.6 s, sys: 2.99 s, total: 46.6 s
+    Wall time: 38.6 s
 %% Cell type:code id: tags:
 ``` python
 %%time
 proj = proj.run(verbose=True)
 print(proj)
 ```
 %%%% Output: stream
     Loading trajectories...
     Smoothing trajectories...
     Interpolating outliers...
     Iterative imputation of ocluded bodyparts...
     Computing distances...
     Computing angles...
     Done!
     deepof analysis of 166 videos
-    CPU times: user 9min 14s, sys: 11.8 s, total: 9min 26s
-    Wall time: 2min 3s
+    CPU times: user 9min, sys: 11.8 s, total: 9min 11s
+    Wall time: 2min 1s
 %% Cell type:markdown id: tags:
 ### 2. Load pretrained deepof model
 %% Cell type:code id: tags:
 ``` python
 coords = proj.get_coords(center="Center", align="Spine_1", align_inplace=True)
 data_prep = coords.preprocess(test_videos=0, window_step=1, window_size=window_size, shuffle=False)[
     0
 ]
 ```
 %% Cell type:code id: tags:
 ``` python
 [i for i in os.listdir(trained_network) if i.endswith("h5")]
 ```
 %%%% Output: execute_result
     ['GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=10_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=9_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=9_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=5_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=10_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=10_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=10_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=10_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=2_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=2_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=2_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
      'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=9_final_weights.h5',
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