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MPIBP-Hummer
BioEM
Commits
1f5be3fc
Commit
1f5be3fc
authored
Oct 01, 2015
by
Pilar Cossio
Browse files
Tuttorial update
parent
8ae18d67
Changes
5
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Tutorial/Euler_Angle_List
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2
0.836000 1.369000 2.640000
-1.375000 0.400000 3.018000
Tutorial_BioEM/BioEM_Manual.pdf
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Tutorial_BioEM/BioEM_Manual.tex
0 → 100644
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Tutorial_BioEM/MODEL_COMPARISION/Param_Input_ModelComparision
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##### Micrograph Parameters #######
NUMBER_PIXELS 224
PIXEL_SIZE 1.32
GRIDPOINTS_ENVELOPE 3
START_ENVELOPE 0.000
END_ENVELOPE 0.001
GRIDPOINTS_PSF_PHASE 3
START_PSF_PHASE 0.004
END_PSF_PHASE 0.024
GRIDPOINTS_PSF_AMP 1
START_PSF_AMP 1.
END_PSF_AMP 1.
MAX_D_CENTER 20
PIXEL_GRID_CENTER 2
###### Sincs its a full atom structure #####
NO_PROJECT_RADIUS
QUATERNIONS
##### Quaterion grid points: #######
USE_QUATERNIONS
##### Constrast transfer integration: #######
CTF_B_FACTOR 100.0 300.5 8
CTF_DEFOCUS 1.0 6.0 5
CTF_AMPLITUDE 0.1 0.1 1
##### Center displacement: #######
DISPLACE_CENTER 10 2
Tutorial_BioEM/Param_Print_MAP
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##### Micrograph Parameters #######
NUMBER_PIXELS 220
PIXEL_SIZE 1.77
BEST_AMP 1
######## Center displacement ################
BEST_DX -10
BEST_DY 5
######## Normalization and Offset ################
BEST_NORM 1
BEST_OFFSET 0
BEST_PHASE 0.00068
BEST_ENVELOPE 0.03
WITHNOISE 5.476
######## Euler Angles ################
BEST_ALPHA -0.464793
BEST_GAMMA -0.409391
BEST_BETA 1.97283
#PRINT_ROTATED_MODELS
######## Using PSF ################
#USE_PSF
BEST_PSF_ENVELOPE 0.0002
BEST_PSF_PHASE 0.004
BEST_PSF_AMP 1
##### Using CTF #######
BEST_CTF_B_FACTOR 200.5
BEST_CTF_DEFOCUS 3.5
BEST_CTF_AMP 0.3
######## ADDING NOISE ###########
WITHNOISE 5.476
########## Printing the 3d Model rotation ###########
PRINT_ROTATED_MODELS
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