Commit 1ebd5f15 authored by lucas_miranda's avatar lucas_miranda
Browse files

Increased default dimensionality of latent space

parent 69196db8
...@@ -133,9 +133,9 @@ class GMVAE: ...@@ -133,9 +133,9 @@ class GMVAE:
defaults = { defaults = {
"bidirectional_merge": "concat", "bidirectional_merge": "concat",
"clipvalue": 1.0, "clipvalue": 0.75,
"dense_activation": "relu", "dense_activation": "relu",
"dense_layers_per_branch": 3, "dense_layers_per_branch": 1,
"dropout_rate": 0.1, "dropout_rate": 0.1,
"learning_rate": 1e-4, "learning_rate": 1e-4,
"units_conv": 64, "units_conv": 64,
......
...@@ -19,11 +19,11 @@ warmup_epochs = [15] ...@@ -19,11 +19,11 @@ warmup_epochs = [15]
warmup_mode = ["sigmoid"] warmup_mode = ["sigmoid"]
losses = ["ELBO"] # , "MMD", "ELBO+MMD"] losses = ["ELBO"] # , "MMD", "ELBO+MMD"]
overlap_loss = [0.1, 0.2, 0.5, 0.75, 1.] overlap_loss = [0.1, 0.2, 0.5, 0.75, 1.]
encodings = [32] # [2, 4, 6, 8, 10, 12, 14, 16] encodings = [16] # [2, 4, 6, 8, 10, 12, 14, 16]
cluster_numbers = [15] # [1, 5, 10, 15, 20, 25] cluster_numbers = [15] # [1, 5, 10, 15, 20, 25]
latent_reg = ["variance"] # ["none", "categorical", "variance", "categorical+variance"] latent_reg = ["variance"] # ["none", "categorical", "variance", "categorical+variance"]
entropy_knn = [10] entropy_knn = [10]
next_sequence_pred_weights = [0.15] next_sequence_pred_weights = [0.0]
phenotype_pred_weights = [0.0] phenotype_pred_weights = [0.0]
rule_based_pred_weights = [0.0] rule_based_pred_weights = [0.0]
window_lengths = [22] # range(11,56,11) window_lengths = [22] # range(11,56,11)
......
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
%load_ext autoreload %load_ext autoreload
%autoreload 2 %autoreload 2
``` ```
   
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
import warnings import warnings
   
warnings.filterwarnings("ignore") warnings.filterwarnings("ignore")
``` ```
   
%% Cell type:markdown id: tags: %% Cell type:markdown id: tags:
   
# deepOF model evaluation # deepOF model evaluation
   
%% Cell type:markdown id: tags: %% Cell type:markdown id: tags:
   
Given a dataset and a trained model, this notebook allows the user to Given a dataset and a trained model, this notebook allows the user to
   
* Load and inspect the different models (encoder, decoder, grouper, gmvaep) * Load and inspect the different models (encoder, decoder, grouper, gmvaep)
* Visualize reconstruction quality for a given model * Visualize reconstruction quality for a given model
* Visualize a static latent space * Visualize a static latent space
* Visualize trajectories on the latent space for a given video * Visualize trajectories on the latent space for a given video
* sample from the latent space distributions and generate video clips showcasing generated data * sample from the latent space distributions and generate video clips showcasing generated data
   
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
import os import os
   
os.chdir(os.path.dirname("../")) os.chdir(os.path.dirname("../"))
``` ```
   
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
import deepof.data import deepof.data
import deepof.utils import deepof.utils
import numpy as np import numpy as np
import pandas as pd import pandas as pd
import re import re
import tensorflow as tf import tensorflow as tf
from collections import Counter from collections import Counter
from sklearn.preprocessing import StandardScaler from sklearn.preprocessing import StandardScaler
   
from sklearn.manifold import TSNE from sklearn.manifold import TSNE
from sklearn.decomposition import PCA from sklearn.decomposition import PCA
from sklearn.discriminant_analysis import LinearDiscriminantAnalysis from sklearn.discriminant_analysis import LinearDiscriminantAnalysis
import umap import umap
   
from ipywidgets import interactive, interact, HBox, Layout, VBox from ipywidgets import interactive, interact, HBox, Layout, VBox
from IPython import display from IPython import display
from matplotlib.animation import FuncAnimation from matplotlib.animation import FuncAnimation
from mpl_toolkits.mplot3d import Axes3D from mpl_toolkits.mplot3d import Axes3D
import matplotlib.pyplot as plt import matplotlib.pyplot as plt
import seaborn as sns import seaborn as sns
import tqdm.notebook as tqdm import tqdm.notebook as tqdm
   
from ipywidgets import interact from ipywidgets import interact
``` ```
   
%% Cell type:markdown id: tags: %% Cell type:markdown id: tags:
   
### 1. Define and run project ### 1. Define and run project
   
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
path = os.path.join("..", "..", "Desktop", "deepof-data", "deepof_single_topview") path = os.path.join("..", "..", "Desktop", "deepof-data", "deepof_single_topview")
trained_network = os.path.join("..", "..", "Desktop", "deepof_trained_weights_280521", "var_annealing") trained_network = os.path.join("..", "..", "Desktop", "deepof_trained_weights_280521", "var_annealing")
exclude_bodyparts = tuple([""]) exclude_bodyparts = tuple([""])
window_size = 22 window_size = 22
batch_size = 32 batch_size = 32
``` ```
   
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
%%time %%time
proj = deepof.data.project( proj = deepof.data.project(
path=path, smooth_alpha=0.999, exclude_bodyparts=exclude_bodyparts, arena_dims=[380], path=path, smooth_alpha=0.999, exclude_bodyparts=exclude_bodyparts, arena_dims=[380],
) )
``` ```
   
%%%% Output: stream %%%% Output: stream
   
CPU times: user 47.3 s, sys: 3.22 s, total: 50.5 s CPU times: user 47.3 s, sys: 3.22 s, total: 50.5 s
Wall time: 42.8 s Wall time: 42.8 s
   
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
%%time %%time
proj = proj.run(verbose=True) proj = proj.run(verbose=True)
print(proj) print(proj)
``` ```
   
%%%% Output: stream %%%% Output: stream
   
Loading trajectories... Loading trajectories...
Smoothing trajectories... Smoothing trajectories...
Interpolating outliers... Interpolating outliers...
Iterative imputation of ocluded bodyparts... Iterative imputation of ocluded bodyparts...
Computing distances... Computing distances...
Computing angles... Computing angles...
Done! Done!
deepof analysis of 166 videos deepof analysis of 166 videos
CPU times: user 16min 22s, sys: 26.6 s, total: 16min 49s CPU times: user 16min 22s, sys: 26.6 s, total: 16min 49s
Wall time: 3min 28s Wall time: 3min 28s
   
%% Cell type:markdown id: tags: %% Cell type:markdown id: tags:
   
### 2. Load pretrained deepof model ### 2. Load pretrained deepof model
   
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
coords = proj.get_coords(center="Center", align="Spine_1", align_inplace=True) coords = proj.get_coords(center="Center", align="Spine_1", align_inplace=True)
data_prep = coords.preprocess(test_videos=0, window_step=1, window_size=window_size, shuffle=False)[ data_prep = coords.preprocess(test_videos=0, window_step=1, window_size=window_size, shuffle=False)[
0 0
] ]
``` ```
   
%% Cell type:code id: tags: %% Cell type:code id: tags:
   
``` python ``` python
[i for i in os.listdir(trained_network) if i.endswith("h5")] [i for i in os.listdir(trained_network) if i.endswith("h5")]
``` ```
   
%%%% Output: execute_result %%%% Output: execute_result
   
['GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5', ['GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=5_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=10_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=25_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=10_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=9_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=9_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=20_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=4_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=9_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=9_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=15_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=3_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.15_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=10_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=6_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=5_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.15_loss=ELBO_loss_warmup=5_warmup_mode=linear_encoding=6_k=15_latreg=variance_entknn=100_run=1_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=25_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=7_final_weights.h5',
'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5', 'GMVAE_input_type=coords_window_size=22_NextSeqPred=0.0_PhenoPred=0.0_RuleBasedPred=0.0_loss=ELBO_loss_warmup=5_warmup_mode=sigmoid_encoding=6_k=15_latreg=variance_entknn=100_run=8_final_weights.h5',
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