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W.}, Journal = {{Trends in Biochemical Sciences}}, Year = {{2015}}, Month = {{JAN}}, Number = {{1}}, Pages = {{49-57}}, Volume = {{40}}, Doi = {{10.1016/j.tibs.2014.10.005}}, ISSN = {{0968-0004}}, Unique-id = {{ISI:000347862900007}} } @InCollection{NEwREV-ELad, Title = {{Cryogenic Electron Microscopy and Single-Particle Analysis}}, Author = {Elmlund, Dominika and Elmlund, Hans}, Booktitle = {{Annual Review of Biochemistry, Vol. 84}}, Year = {{2015}}, Editor = {{Kornberg, RD}}, Pages = {{499-517}}, Series = {{Annual Review of Biochemistry}}, Volume = {{84}}, Doi = {{10.1146/annurev-biochem-060614-034226}}, ISBN = {{978-0-8243-0884-1}}, ISSN = {{0066-4154}}, Orcid-numbers = {{Elmlund, Hans/0000-0002-6992-0601}}, Researcherid-numbers = {{Elmlund, Hans/}}, Unique-id = {{ISI:000355765300019}} } @article{Penczek_2010, Author = {Penczek, Pawel A.}, Title = {{Image Restoration in Cryo-Electron Microscopy}}, Journal = {{Methods in Enzymology}}, Year = {{2010}}, Volume = {{482}}, Pages = {{35-72}} } @Article{AllegrettiNAT2015, Title = {{Horizontal membrane-intrinsic alpha-helices in the stator a-subunit of an F-type ATP synthase}}, Author = {Allegretti, Matteo and Klusch, Niklas and Mills, Deryck J. and Vonck, Janet and Kuehlbrandt, Werner and Davies, Karen M.}, Journal = {{Nature}}, Year = {{2015}}, Month = {{MAY 14}}, Number = {{7551}}, Pages = {{237+}}, Volume = {{521}}, Abstract = {{ATP, the universal energy currency of cells, is produced by F-type ATP synthases, which are ancient, membrane-bound nanomachines. F-type ATP synthases use the energy of a transmembrane electrochemical gradient to generate ATP by rotary catalysis. Protons moving across the membrane drive a rotor ring composed of 8-15 c-subunits(1). A central stalk transmits the rotation of the c-ring to the catalytic F-1 head, where a series of conformational changes results in ATP synthesis(2). A key unresolved question in this fundamental process is how protons pass through the membrane to drive ATP production. Mitochondrial ATP synthases form V-shaped homodimers in cristae membranes(3). Here we report the structure of a native and active mitochondrial ATP synthase dimer, determined by single-particle electron cryomicroscopy at 6.2 angstrom resolution. Our structure shows four long, horizontal membrane-intrinsic alpha-helices in the a-subunit, arranged in two hairpins at an angle of approximately 70 degrees relative to the c-ring helices. It has been proposed that a strictly conserved membrane-embedded arginine in the a-subunit couples proton translocation to c-ring rotation(4). A fit of the conserved carboxy-terminal a-subunit sequence places the conserved arginine next to a proton-binding c-subunit glutamate. The map shows a slanting solvent-accessible channel that extends from the mitochondrial matrix to the conserved arginine. Another hydrophilic cavity on the lumenal membrane surface defines a direct route for the protons to an essential histidine-glutamate pair(5). Our results provide unique new insights into the structure and function of rotary ATP synthases and explain how ATP production is coupled to proton translocation.}}, Address = {{MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND}}, Affiliation = {{Kuhlbrandt, W (Reprint Author), Max Planck Inst Biophys, Dept Struct Biol, Max von Laue Str 3, D-60438 Frankfurt, Germany. Allegretti, Matteo; Klusch, Niklas; Mills, Deryck J.; Vonck, Janet; Kuehlbrandt, Werner; Davies, Karen M., Max Planck Inst Biophys, Dept Struct Biol, D-60438 Frankfurt, Germany.}}, Author-email = {{werner.kuehlbrandt@biophys.mpg.de karen.davies@biophys.mpg.de}}, Cited-references = {{ABRAHAMS JP, 1994, NATURE, V370, P621, DOI 10.1038/370621a0. ALLEGRETTI M, 2014, ELIFE, V0003. Andrade MA, 2001, J MOL BIOL, V309, P1, DOI 10.1006/jmbi.2001.4624. Angevine CM, 2003, J BIOL CHEM, V278, P6066, DOI 10.1074/jbc.M210199200. Atteia A, 2000, EUR J BIOCHEM, V267, P2850, DOI 10.1046/j.1432-1327.2000.01288.x. Cain B. D., 1988, J BIOL CHEM, V263, P6602. CAREAGA CL, 1992, J MOL BIOL, V226, P1219, DOI 10.1016/0022-2836(92)91063-U. Chen SX, 2013, ULTRAMICROSCOPY, V135, P24, DOI 10.1016/j.ultramic.2013.06.004. Davies KM, 2012, P NATL ACAD SCI USA, V109, P13602, DOI 10.1073/pnas.1204593109. Davies KM, 2011, P NATL ACAD SCI USA, V108, P14121, DOI 10.1073/pnas.1103621108. 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Castillo-Hernandez provided computer support. This work was funded by the Max Planck Society (M.A., N.K., D.J.M., J.V., K.M.D., W.K.) and the Deutsche Forschungsgemeinschaft Cluster of Excellence Frankfurt `Macromolecular Complexes' (K.M.D., W.K.).}}, ISSN = {{0028-0836}}, Journal-iso = {{Nature}}, Keywords-plus = {{ESSENTIAL ARGININE RESIDUE; POLYTOMELLA SP; CROSS-LINKING; C-RING; CHLAMYDOMONAS-REINHARDTII; ELECTRON-MICROSCOPY; F0F1-ATP SYNTHASE; PROTON CHANNEL; CRYO-EM; RESOLUTION}}, Language = {{English}}, Number-of-cited-references = {{45}}, Publisher = {{NATURE PUBLISHING GROUP}}, Research-areas = {{Science \& Technology - Other Topics}}, Times-cited = {{1}}, Type = {{Article}}, Unique-id = {{ISI:000354377800062}}, Web-of-science-categories = {{Multidisciplinary Sciences}} } @Article{Yershova2010, Title = {{Generating Uniform Incremental Grids on SO(3) Using the Hopf Fibration}}, Author = {Yershova, Anna and Jain, Swati and LaValle, Steven M. and Mitchell, Julie C.}, Journal = {{Int. J. Robot. 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